Optical techniques for cervical neoplasia detection

• Tatiana Novikova

Beilstein J. Nanotechnol. 2017, 8, 1844–1862, doi:10.3762/bjnano.8.186

• increased number of false positive results. Gu et al. [53] suggested using fluorescence-lifetime imaging microscopy (FLIM) on haematoxylin and eosin (H&E) stained histological cuts of cervical tissue and a neural network classifier for the automated diagnosis of CIN lesions. This technique can overcome the

Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques

• Anja Ostrowski,
• Daniel Nordmeyer,
• Alexander Boreham,
• Cornelia Holzhausen,
• Lars Mundhenk,
• Christina Graf,
• Martina C. Meinke,
• Annika Vogt,
• Sabrina Hadam,
• Jürgen Lademann,
• Eckart Rühl,
• Ulrike Alexiev and
• Achim D. Gruber

Beilstein J. Nanotechnol. 2015, 6, 263–280, doi:10.3762/bjnano.6.25

• well-established methods, such as standard light, fluorescence, transmission electron and scanning electron microscopy as well as more recent developments, such as light and electron microscopic autoradiography, fluorescence lifetime imaging, spectral imaging and linear unmixing, superresolution

• these techniques should therefore respect their specific merits and limitations as no single approach combines all desired properties. Keywords: fluorescence lifetime imaging; fluorescence microscopy; histopathology; light microscopic autoradiography; structured illumination microscopy; Introduction

• which have already been reviewed by others from a different angle [20][34][35]. Furthermore, we will highlight and introduce new and more advanced techniques including light and electron microscopic autoradiography, fluorescence lifetime imaging, spectral imaging and linear unmixing, superresolution

Synthesis and characterization of fluorescence-labelled silica core-shell and noble metal-decorated ceria nanoparticles

• Rudolf Herrmann,
• Markus Rennhak and
• Armin Reller

Beilstein J. Nanotechnol. 2014, 5, 2413–2423, doi:10.3762/bjnano.5.251

• ]. Fluorescence lifetime imaging microscopy (FLIM) on CeO2 agglomerates (50 ± 5 nm diameter of the circumscribed sphere) with and without platinum decoration did not show any difference between both samples. We can therefore conclude that there is no efficient energy transfer between metal and fluorescence dye